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Stop Animal Exploitation NOW!
S. A. E. N.
"Exposing the truth to wipe out animal experimentation"

Articles and Reports

Alternatives to Animal Research and Testing

Alternatives to Ascites Production of Monoclonal Antibodies
by John McArdle, Ph.D.
Alternatives Research and Development Foundation
Eden Prairie, Minnesota
www.nal.usda.gov/awic/newsletters/v8n3/8n3mcard.htm

Table 1. Antibody production in different bioreactor systems

Table 1. Antibody production in different bioreactor systems (Adapted from Stoll et al., 1995 (24)).
System Maximum
Concentration
Productivity Required for the
production of 1 g
In vivo 2200 2 180 mice
In vitro
  T-flask 42 7 400 T-flasks
  Stirred-tank bioreactor, batch 47 180a 39 days
  Stirred-tank bioreactor, fed-batch 120 250a 28 days
  Hollow fiber reactor 1600 1400 26; 5 daysb
aThe total cycle time (innoculation, culture and cleaning) was taken into account.
bFirst number is for first gram; second number is for second gram.

Go on to Table 2. Comparisons of different monoclonal production methods
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Alternatives to Ascites Production of Monoclonal Antibodies
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