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Stop Animal Exploitation NOW!
S. A. E. N.
"Exposing the truth to wipe out animal experimentation"

Articles and Reports

Alternatives to Animal Research and Testing

Alternatives to Ascites Production of Monoclonal Antibodies
by John McArdle, Ph.D.
Alternatives Research and Development Foundation
Eden Prairie, Minnesota
www.nal.usda.gov/awic/newsletters/v8n3/8n3mcard.htm

Table 2. Comparisons of different monoclonal production methods

Table 2. Comparisons of different monoclonal production methods. (Adapted from Hendriksen et al., 1996 (6); Kamp amd de Leeu 1996 (11)).
Production System Scale Volume (ml) Concentration (mg/ml) Production Time
(weeks)a
Estimated Cost (per gram) Quality
Ascites (in vivo) 20-250 mg 5-10 < 20 2-3 1000-6000 Low
Stir growth   100-2500 0.01-0.1 2-3   High
Dialysis membrane < 50 mg 10-25 0.1-1.5 2-5   High
Roller bottles < 2 g 100-2000 0.01-0.2 2-6 2000-6000 High
Hollow fiber 0.15-30g 25-1000 0.2-0.3 3-12 2700-4000 High
Fermentor 2-100g < 2000 liters 0.05-0.5 2-12 1000-6000 High
aExcluding immunization, including up-scaling cell cultures.

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