Brooks Air Force Base

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Brooks Air Force Base, TX

Protocol - 2003-07 - Development of a Chronic Wound Eschar Model in Swine



Development of a Chronic Wound Eschar Model in Swine for the Evaluation of Debridement Modalities


Research Category:

M6: Combat Casualty Care


FY: 2003 Funding (in dollars):



Responsible Organization:


Primary Contact:

Public Affairs Office


Brooks City-Base






Performing Organization:

See Responsible Organization Information












The work is being performed under a Cooperative Research and Development Agreement (CRADA) with a commercial health care products company (Healthpoint Ltd). There are two objectives in this project 1. To produce a reproducible model of necrotic wounds at Brooks City Base. a. One specific for burns b. One excisional wounds.A necrosing agent is required to induce eschar formation (in non-thermal injuries). 2. To determine whether trichrome stain (or Martius Yellow - Scarlett Blue stain) is effective at determining the depth of necrotic tissue in both thermal and excisional wounds.



Several publications describe the benefits of early debridement of wounded skin. Early debridement prevents numerous wound complications and facilitates rapid wound repair. In many battlefield circumstances, surgical debridement is not an option. However, enzymatic debridement, if done effectively, may be useful in promoting the healing process. In order to discover and demonstrate the effectiveness of enzymatic debriding agents, it is necessary to develop a model to test these materials in vivo. This protocol describes the route to evaluating several different methods to arrive at a well-defined and standardized model for debridement studies. This project is designed to develop an animal model of eschar debridement. Wounds will be created on the backs of domestic Yorkshire cross swine and eschar formation will be allowed to proceed naturally, or may be assisted by application of dermal penetration enhancers or necrosing agents. The model, once developed, will be used to assess the efficacy of novel debriding agents. Model development will be divided into two separate phases. The first phase will examine the formation of eschar created by thermally induced wounds and wounds created by full-thickness excision and which will receive additional treatment with common pharmaceutical agents that are designed to maximize skin penetration for the delivery of active agents to deeper tissues, this penetrating agent will be used as a necrosing agent to create a non-thermal eschar. After the completion of this phase, models will be chosen for the thermal and non-thermal wounding methods that appear to provide the best eschar. The second phase will establish the reproducibility of the chosen method from phase one of the study.

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Rats, mice, birds, amphibians and other animals have been excluded from coverage by the Animal Welfare Act. Therefore research facility reports do not include these animals. As a result of this situation, a blank report, or one with few animals listed, does not mean that a facility has not performed experiments on non-reportable animals. A blank form does mean that the facility in question has not used covered animals (primates, dogs, cats, rabbits, guinea pigs, hamsters, pigs, sheep, goats, etc.). Rats and mice alone are believed to comprise over 90% of the animals used in experimentation. Therefore the majority of animals used at research facilities are not even counted.

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