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Stop Animal
Exploitation NOW!
S. A. E. N.
"Exposing the truth to wipe
out animal experimentation"
 Articles and
Reports
Alternatives to Animal Research and Testing
Alternatives to Ascites Production of Monoclonal Antibodies
by John McArdle, Ph.D.
Alternatives Research and Development Foundation
Eden Prairie, Minnesota
www.nal.usda.gov/awic/newsletters/v8n3/8n3mcard.htm
Table 1. Antibody production in different bioreactor systems
| Table 1. Antibody production in
different bioreactor systems (Adapted from Stoll et al., 1995 (24)). |
| System |
Maximum
Concentration |
Productivity |
Required for the
production of 1 g |
| In vivo |
2200 |
2 |
180 mice |
| In vitro |
| T-flask |
42 |
7 |
400 T-flasks |
| Stirred-tank bioreactor, batch |
47 |
180a |
39 days |
| Stirred-tank bioreactor, fed-batch |
120 |
250a |
28 days |
| Hollow fiber reactor |
1600 |
1400 |
26; 5 daysb |
aThe total cycle time (innoculation,
culture and cleaning) was taken into account.
bFirst number is for first gram; second number is for
second gram. |
Go on to
Table 2. Comparisons of
different monoclonal production methods
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of Monoclonal Antibodies
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