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Air Force Research Laboratory, Wright-Patterson AFB, OH

DOD Funding of Animal Cruelty 2005: Continuing Development of a Tissue Based Biosensor (TBB) using Rats, Mice and Fish

Title: Continuing Development of a Tissue Based Biosensor (TBB) using Rats, Mice and Fish

Research Category:

FY: 2005 Funding (in dollars): 60,000

Primary Contact: See Responsible Organization Information
City: Wright-Patterson AFB
State: OH
Zip: 45433-7902


Objective: To develop a tissue-based biosensor (a biosensor using living tissue) with the capability of responding to a wide range of chemical toxicants or stressors.

Approach: The protocol describes the use of living central nervous system (CNS) tissue slices from mouse, rat or bluegill sunfish for application to existing 64-electrode microarrays that measure a wide range of changes in the electrical responsivity of tissue slices. It is hypothesized that living tissue slices adhered to (acute applications) or cultured on (long-term applications) microarrays can be successfully used to recognize and/or identify chemicals and other stressors with the capacity for human risk. It is further hypothesized that such tissue based biosensors can be cost-effectively used to detect chemical toxicants and stressors at concentrations well below those sufficient to induce human histopathology.

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Rats, mice, birds, amphibians and other animals have been excluded from coverage by the Animal Welfare Act. Therefore research facility reports do not include these animals. As a result of this situation, a blank report, or one with few animals listed, does not mean that a facility has not performed experiments on non-reportable animals. A blank form does mean that the facility in question has not used covered animals (primates, dogs, cats, rabbits, guinea pigs, hamsters, pigs, sheep, goats, etc.). Rats and mice alone are believed to comprise over 90% of the animals used in experimentation. Therefore the majority of animals used at research facilities are not even counted.

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